Resolve photons in space and in time at once — a megapixel field at 40 ps, with no scanning.
A confocal FLIM system builds an image one point at a time. LINCam does not scan: the whole field is live at once, and each detected photon is recorded with its position on the sensor and its arrival time relative to the excitation pulse.
What comes out is not a picture but a photon list. You decide afterwards how to bin it — in space, in time, or by lifetime component.
LINCam mounts on a standard camera port. Four steps, no optical redesign, no scanner to synchronise.
Wherever lifetime is the observable and the sample will not hold still long enough for a scanner, a position-sensitive detector changes what is measurable.
Large scale FLIM by optical sectioning of rat embryo. When the size matters.
By using the metabolite NADH as an intrinsic marker for glycolysis, the dynamics of individual cells can be monitored and their interactions studied.
Metal induced energy transfer (MIET) is a nanoscopy technique to measure distances from fluorescent molecules to a metal film. Here we present MIET acquisition using wide-field and TIRF fluorescence lifetime imaging microscopy (FLIM) equipped with LINCam.
Overcoming the challenge of multilabel imaging with lifetime chromophore separation.
Every LINCam ships with Preview, the FLIM evaluation kit. The Python module and the MATLAB toolbox read the same photon files.
Tell us about your setup and what you need to measure.